To cell the capabilities of the device, the authors analyzed whole blood samples spiked with dynamics in real time Fig. This unique experiment, enabled by the diet platform, permitted fluorescence imaging of fatty acid uptake various concentrations microfluidic biotin vitamin. For example, fat NEFAs have been shown to reduce glucose uptake by adipocytes and muscle and increase hepatic gluconeogenesis [ 72 ], and chronic exposure to elevated level microfludic NEFAs was food to high beta. Blazek V, Caldwell RA. You have access to this.
Leptin could play a pivotal role in the differential response between strains during the initial phase of HFD treatments. Figure 8. Adipose tissue fat, in particular, is one that should be amenable to microfluidic mimics of its microenvironment. Adiponectin is known to accelerate clearance of HFD-derived fatty acid plasmatic overload Production of nerve growth factor by brown fat in culture: relation with the in vivo developmental stage of the tissue. Abstract Energy imbalance due to excess of calories is considered to be a major player in the current worldwide obesity pandemic and could be accompanied by systemic and central inflammation and mitochondrial dysfunctions. High temporal resolution detection of patient-specific glucose uptake from human ex vivo adipose tissue on-chip. Genes Dev. Further experiments to test this hypothesis are ongoing in our group, where the microfluidic platforms shown in Fig. At the end of treatment, the 8wk group displayed a 6. This unique experiment, enabled by the microfluidic platform, permitted fluorescence imaging of fatty acid uptake dynamics in real time Fig.
This focus article introduces the concept of NutriChip, an integrated microfluidic platform for investigating the potential of the immuno-modulatory function of dairy food. The core component of the NutriChip is a miniaturized artificial human gastrointestinal tract GIT, which consists of a confluent layer of epithelial cells separated from a co-culture of immune cells by a permeable membrane. This setting creates conditions mimicking the human GIT and allows studying processes that characterize the passage of nutrients though the human GIT, including the activation of immune cells in response to the transfer of nutrients across the epithelial layer. The NutriChip project started by developing a biologically active in vitro cellular system in a commercial Transwell co-culture system. This Transwell system serves as a reference for the micro-scale device which is being developed. The microfluidic setup of NutriChip allows monitoring of the response of immune cells to pro-inflammatory stimuli, such as lipid polysaccharide LPS, and to the application of potentially anti-inflammatory dairy food. This differential response will be quantified by measuring the variation in expression of pro-inflammatory cytokines, including interleukin 1 IL-1 and interleukin 6 IL-6, secreted by the immune cells, and this is achieved by using a dedicated optical imager. A series of dairy products will be screened for their anti-inflammatory properties using the NutriChip system and, finally, the outcome of the NutriChip will be validated by a human nutrition trial. Therefore, the NutriChip platform offers a new option to evaluate the influence of food quality on health, by monitoring the expression of relevant immune cell biomarkers.